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abberior instruments stedycon sted microscope
Stedycon Sted Microscope, supplied by abberior instruments, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/stedycon/stedycon+sted+microscope/pm40663602-313-6-9
Average 90 stars, based on 1 article reviews
stedycon sted microscope - by Bioz Stars, 2026-10
90/100 stars

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Related Articles

other:

Article Title: Control of epithelial tissue organization by mRNA localization.
Article Snippet: The gross tissue morphology of each tissue was visualized by H&E staining (abcam; ab245880).

Microscopy:

Article Title: Genetically encoded intrabody probes for labeling and manipulating AMPA-type glutamate receptors
Article Snippet: .. STED super-resolution images were acquired using the commercial Abberior STEDYCON addition to an Olympus confocal microscope equipped with the following: an Olympus UPLXAPO100XO 100X magnification and 1.45 NA. objective, 4 excitation lasers; 405(cw), 485 nm(pulsed), 561 nm(pulsed), 640 nm(pulsed), 4 corresponding single-photon counting APD detectors (avalanche photodiodes), and a 775 nm laser for stimulated emission depletion. .. Samples were stained using secondary antibodies labeled with Abberior STAR RED, STAR 635 and/or STAR ORANGE, STAR 580, and STAR 460 L and mounted for 24+ hours in prolong gold mounting medium.

Article Title: Genetically encoded intrabody probes for labeling and manipulating AMPA-type glutamate receptors.
Article Snippet: .. STED Imaging and Analysis STED super-resolution images were acquired using the commercial Abberior STEDYCON addition to an Olympus confocal microscope equipped with the following: an Olympus UPLXAPO100XO 100X magnification and 1.45 NA. objective, 4 excitation lasers; 405(cw), 485 nm(pulsed), 561 nm(pulsed), 640 nm(pulsed), 4 corresponding single-photon counting APD detectors (avalanche photodiodes), and a 775 nm laser for stimulated emission depletion. ..

Staining:

Article Title: Investigation of neuromodulation of the endbulb of Held synapse in the cochlear nucleus by serotonin and norepinephrine
Article Snippet: .. Two-color STED images of cryosections stained for monoamine transporters were acquired using a STEDYCON (Abberior Instruments GmbH, Göttingen, Germany) with a 100× oil immersion objective. .. Images of the monoamine receptor preparations were taken with Zeiss confocal laser scanning microscopes 780 and 880 with 40× or 100× oil immersion objectives.

Imaging:

Article Title: Genetically encoded intrabody probes for labeling and manipulating AMPA-type glutamate receptors.
Article Snippet: .. STED Imaging and Analysis STED super-resolution images were acquired using the commercial Abberior STEDYCON addition to an Olympus confocal microscope equipped with the following: an Olympus UPLXAPO100XO 100X magnification and 1.45 NA. objective, 4 excitation lasers; 405(cw), 485 nm(pulsed), 561 nm(pulsed), 640 nm(pulsed), 4 corresponding single-photon counting APD detectors (avalanche photodiodes), and a 775 nm laser for stimulated emission depletion. ..

Article Title: Oligodendrocyte Slc48a1 (Hrg1) encodes a functional heme transporter required for myelin integrity
Article Snippet: For stimulated emission depletion (STED) microscopy, standard immunostaining protocols were used with the exception of 10% (v/v) normal goat serum in replacement of donkey serum, incubation of primary antibodies for 18 h, omission of Hoechst and incubating with STED compatible secondary antibodies (Table ) (Abberior Instruments GmBH, DE) for 2 h at RT and doubling of the washing steps at all stages. .. STED imaging was conducted using a Zeiss Axioscope with STEDYcon (Abberior Instruments GmBH, DE) using a 100X oil immersion objective. .. Mouse smFISH was performed using the RNAScope LS Multiplex Assay (Biotechne, UK) as previously described (Bayraktar et al., ).

Inverted Epifluorescence:

Article Title: Expression of alpha smooth muscle actin decreases with ageing and increases upon lumen obstruction in mouse brain pericytes.
Article Snippet: As a primary antibody, anti-γ-actin (Abcam; cat. no. ab123034) and vWF (Abcam; cat. no. ab6994) were used in a dilution of 1:50, while the secondary antibodies were anti-mouse Alexa Fluor 647 (Thermo Fisher Scientific; cat. no. A32787) and anti-rabbit Alexa Fluor 488 (Thermo Fisher Scientific; cat. no. A32790) diluted to 1:500. .. Samples were analysed using a STEDYCON (Abberior Instruments, Göttingen, Germany) built on an Axio Observer Z1 inverted epifluorescence microscope (Zeiss, Oberkochen, Germany) equipped with an alpha Plan-Apochromat 100 × /1.46 oil immersion objective. ..

Article Title: Expression of alpha smooth muscle actin decreases with ageing and increases upon lumen obstruction in mouse brain pericytes
Article Snippet: As a primary antibody, anti-γ-actin (Abcam; cat. no. ab123034) and vWF (Abcam; cat. no. ab6994) were used in a dilution of 1:50, while the secondary antibodies were anti-mouse Alexa Fluor 647 (Thermo Fisher Scientific; cat. no. A32787) and anti-rabbit Alexa Fluor 488 (Thermo Fisher Scientific; cat. no. A32790) diluted to 1:500. .. Samples were analysed using a STEDYCON (Abberior Instruments, Göttingen, Germany) built on an Axio Observer Z1 inverted epifluorescence microscope (Zeiss, Oberkochen, Germany) equipped with an alpha Plan-Apochromat 100 × /1.46 oil immersion objective . ..



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Image Search Results


(A) Representative FACS plot of cell sorting strategy to isolate purified oxLDL + and oxLDL - neutrophils. ( B ) Reactome pathway analysis of proteins increased in oxLDL + subset in the proteomics data. ( C ) Heatmap of selected differentially expressed proteins in oxLDL - and oxLDL + neutrophil populations. ( D ) 100x STED immunofluorescent imaging of peritoneal cells isolated from zymosan and oxLDL-Dil IP-injected mice and stained for phalloidin. Cells were also stained and checked for Ly6G expression, which is not shown for clarity of phalloidin stain. Representative oxLDL + (left) and oxLDL - (right) cells are shown. ( E ) In vitro oxLDL-Dil uptake in bone marrow enriched neutrophils treated with cytochalasin D. ( F ) Transwell migration assay of oxLDL - and oxLDL + neutrophils sorted from peritoneal cells following zymosan and oxLDL-Dil IP injection. ( E-F ) All symbols represent independent experiments. Data are expressed as mean ± SEM. Statistical differences were calculated by one-way ANOVA for F and two-way ANOVA for G. * ≤ 0.05, ** ≤ 0.01,***≤0.001

Journal: bioRxiv

Article Title: Neutrophil TLR2 signaling promotes lipid accumulation and vascular plaque growth

doi: 10.1101/2025.07.09.663961

Figure Lengend Snippet: (A) Representative FACS plot of cell sorting strategy to isolate purified oxLDL + and oxLDL - neutrophils. ( B ) Reactome pathway analysis of proteins increased in oxLDL + subset in the proteomics data. ( C ) Heatmap of selected differentially expressed proteins in oxLDL - and oxLDL + neutrophil populations. ( D ) 100x STED immunofluorescent imaging of peritoneal cells isolated from zymosan and oxLDL-Dil IP-injected mice and stained for phalloidin. Cells were also stained and checked for Ly6G expression, which is not shown for clarity of phalloidin stain. Representative oxLDL + (left) and oxLDL - (right) cells are shown. ( E ) In vitro oxLDL-Dil uptake in bone marrow enriched neutrophils treated with cytochalasin D. ( F ) Transwell migration assay of oxLDL - and oxLDL + neutrophils sorted from peritoneal cells following zymosan and oxLDL-Dil IP injection. ( E-F ) All symbols represent independent experiments. Data are expressed as mean ± SEM. Statistical differences were calculated by one-way ANOVA for F and two-way ANOVA for G. * ≤ 0.05, ** ≤ 0.01,***≤0.001

Article Snippet: For super resolution imaging, slides were imaged using a four detector Abberior STEDYCON STED unit.

Techniques: FACS, Purification, Imaging, Isolation, Injection, Staining, Expressing, In Vitro, Transwell Migration Assay