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abberior instruments stedycon sted microscope
Stedycon Sted Microscope, supplied by abberior instruments, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/stedycon/stedycon+sted+microscope/pm40663602-313-6-9
Average 90 stars, based on 1 article reviews
stedycon sted microscope - by Bioz Stars, 2026-09
90/100 stars

Images

Related Articles

Microscopy:

Article Title: Control of epithelial tissue organization by mRNA localization.
Article Snippet: Images were acquired using the Carl Zeiss Zen 2.3 software and analyzed using the HALO image analysis platform (Indica Labs). .. For Stimulated Emission Depletion (STED) microscopy, images were recorded by STEDYCON (Abberior Instruments) assembled on Eclipse Ti2 inverted microscope (Nikon Inc.) and 100×, NA 1.45 Plan Apo objective. ..

Article Title: Genetically encoded intrabody probes for labeling and manipulating AMPA-type glutamate receptors
Article Snippet: .. STED super-resolution images were acquired using the commercial Abberior STEDYCON addition to an Olympus confocal microscope equipped with the following: an Olympus UPLXAPO100XO 100X magnification and 1.45 NA. objective, 4 excitation lasers; 405(cw), 485 nm(pulsed), 561 nm(pulsed), 640 nm(pulsed), 4 corresponding single-photon counting APD detectors (avalanche photodiodes), and a 775 nm laser for stimulated emission depletion. .. Samples were stained using secondary antibodies labeled with Abberior STAR RED, STAR 635 and/or STAR ORANGE, STAR 580, and STAR 460 L and mounted for 24+ hours in prolong gold mounting medium.

Article Title: Genetically encoded intrabody probes for labeling and manipulating AMPA-type glutamate receptors.
Article Snippet: .. STED Imaging and Analysis STED super-resolution images were acquired using the commercial Abberior STEDYCON addition to an Olympus confocal microscope equipped with the following: an Olympus UPLXAPO100XO 100X magnification and 1.45 NA. objective, 4 excitation lasers; 405(cw), 485 nm(pulsed), 561 nm(pulsed), 640 nm(pulsed), 4 corresponding single-photon counting APD detectors (avalanche photodiodes), and a 775 nm laser for stimulated emission depletion. ..

Inverted Microscopy:

Article Title: Control of epithelial tissue organization by mRNA localization.
Article Snippet: Images were acquired using the Carl Zeiss Zen 2.3 software and analyzed using the HALO image analysis platform (Indica Labs). .. For Stimulated Emission Depletion (STED) microscopy, images were recorded by STEDYCON (Abberior Instruments) assembled on Eclipse Ti2 inverted microscope (Nikon Inc.) and 100×, NA 1.45 Plan Apo objective. ..

Staining:

Article Title: Investigation of neuromodulation of the endbulb of Held synapse in the cochlear nucleus by serotonin and norepinephrine
Article Snippet: .. Two-color STED images of cryosections stained for monoamine transporters were acquired using a STEDYCON (Abberior Instruments GmbH, Göttingen, Germany) with a 100× oil immersion objective. .. Images of the monoamine receptor preparations were taken with Zeiss confocal laser scanning microscopes 780 and 880 with 40× or 100× oil immersion objectives.

Imaging:

Article Title: Genetically encoded intrabody probes for labeling and manipulating AMPA-type glutamate receptors.
Article Snippet: .. STED Imaging and Analysis STED super-resolution images were acquired using the commercial Abberior STEDYCON addition to an Olympus confocal microscope equipped with the following: an Olympus UPLXAPO100XO 100X magnification and 1.45 NA. objective, 4 excitation lasers; 405(cw), 485 nm(pulsed), 561 nm(pulsed), 640 nm(pulsed), 4 corresponding single-photon counting APD detectors (avalanche photodiodes), and a 775 nm laser for stimulated emission depletion. ..

Article Title: Oligodendrocyte Slc48a1 (Hrg1) encodes a functional heme transporter required for myelin integrity
Article Snippet: For stimulated emission depletion (STED) microscopy, standard immunostaining protocols were used with the exception of 10% (v/v) normal goat serum in replacement of donkey serum, incubation of primary antibodies for 18 h, omission of Hoechst and incubating with STED compatible secondary antibodies (Table ) (Abberior Instruments GmBH, DE) for 2 h at RT and doubling of the washing steps at all stages. .. STED imaging was conducted using a Zeiss Axioscope with STEDYcon (Abberior Instruments GmBH, DE) using a 100X oil immersion objective. .. Mouse smFISH was performed using the RNAScope LS Multiplex Assay (Biotechne, UK) as previously described (Bayraktar et al., ).

Inverted Epifluorescence:

Article Title: Expression of alpha smooth muscle actin decreases with ageing and increases upon lumen obstruction in mouse brain pericytes.
Article Snippet: As a primary antibody, anti-γ-actin (Abcam; cat. no. ab123034) and vWF (Abcam; cat. no. ab6994) were used in a dilution of 1:50, while the secondary antibodies were anti-mouse Alexa Fluor 647 (Thermo Fisher Scientific; cat. no. A32787) and anti-rabbit Alexa Fluor 488 (Thermo Fisher Scientific; cat. no. A32790) diluted to 1:500. .. Samples were analysed using a STEDYCON (Abberior Instruments, Göttingen, Germany) built on an Axio Observer Z1 inverted epifluorescence microscope (Zeiss, Oberkochen, Germany) equipped with an alpha Plan-Apochromat 100 × /1.46 oil immersion objective. ..

Article Title: Expression of alpha smooth muscle actin decreases with ageing and increases upon lumen obstruction in mouse brain pericytes
Article Snippet: As a primary antibody, anti-γ-actin (Abcam; cat. no. ab123034) and vWF (Abcam; cat. no. ab6994) were used in a dilution of 1:50, while the secondary antibodies were anti-mouse Alexa Fluor 647 (Thermo Fisher Scientific; cat. no. A32787) and anti-rabbit Alexa Fluor 488 (Thermo Fisher Scientific; cat. no. A32790) diluted to 1:500. .. Samples were analysed using a STEDYCON (Abberior Instruments, Göttingen, Germany) built on an Axio Observer Z1 inverted epifluorescence microscope (Zeiss, Oberkochen, Germany) equipped with an alpha Plan-Apochromat 100 × /1.46 oil immersion objective . ..



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Image Search Results


(A) Representative FACS plot of cell sorting strategy to isolate purified oxLDL + and oxLDL - neutrophils. ( B ) Reactome pathway analysis of proteins increased in oxLDL + subset in the proteomics data. ( C ) Heatmap of selected differentially expressed proteins in oxLDL - and oxLDL + neutrophil populations. ( D ) 100x STED immunofluorescent imaging of peritoneal cells isolated from zymosan and oxLDL-Dil IP-injected mice and stained for phalloidin. Cells were also stained and checked for Ly6G expression, which is not shown for clarity of phalloidin stain. Representative oxLDL + (left) and oxLDL - (right) cells are shown. ( E ) In vitro oxLDL-Dil uptake in bone marrow enriched neutrophils treated with cytochalasin D. ( F ) Transwell migration assay of oxLDL - and oxLDL + neutrophils sorted from peritoneal cells following zymosan and oxLDL-Dil IP injection. ( E-F ) All symbols represent independent experiments. Data are expressed as mean ± SEM. Statistical differences were calculated by one-way ANOVA for F and two-way ANOVA for G. * ≤ 0.05, ** ≤ 0.01,***≤0.001

Journal: bioRxiv

Article Title: Neutrophil TLR2 signaling promotes lipid accumulation and vascular plaque growth

doi: 10.1101/2025.07.09.663961

Figure Lengend Snippet: (A) Representative FACS plot of cell sorting strategy to isolate purified oxLDL + and oxLDL - neutrophils. ( B ) Reactome pathway analysis of proteins increased in oxLDL + subset in the proteomics data. ( C ) Heatmap of selected differentially expressed proteins in oxLDL - and oxLDL + neutrophil populations. ( D ) 100x STED immunofluorescent imaging of peritoneal cells isolated from zymosan and oxLDL-Dil IP-injected mice and stained for phalloidin. Cells were also stained and checked for Ly6G expression, which is not shown for clarity of phalloidin stain. Representative oxLDL + (left) and oxLDL - (right) cells are shown. ( E ) In vitro oxLDL-Dil uptake in bone marrow enriched neutrophils treated with cytochalasin D. ( F ) Transwell migration assay of oxLDL - and oxLDL + neutrophils sorted from peritoneal cells following zymosan and oxLDL-Dil IP injection. ( E-F ) All symbols represent independent experiments. Data are expressed as mean ± SEM. Statistical differences were calculated by one-way ANOVA for F and two-way ANOVA for G. * ≤ 0.05, ** ≤ 0.01,***≤0.001

Article Snippet: For super resolution imaging, slides were imaged using a four detector Abberior STEDYCON STED unit.

Techniques: FACS, Purification, Imaging, Isolation, Injection, Staining, Expressing, In Vitro, Transwell Migration Assay